発表論文等

2024

1. Flower color modification in Torenia fournieri by genetic engineering of betacyanin pigments. Nishihara, M.,  Hirabuchi, A., Teshima, T., Uesugi, S., Takahashi, H. BMC Plant Biology  24: 614 (link)

2. Applications of the wheat germ cell-free protein synthesis system in plant biochemical studies. Nemoto K.  Plant Biotechnology (in press).

2023

1.  Identification of a novel viral factor inducing tumorous symptoms by disturbing vascular development in planta. Atsumi, G., Naramoto, S., Nishihara, M., Nakatsuka, T., Tomita, R., Matsushita, Y., Hoshi, N., Shirakawa, A., Kobayashi, K., Fukuda, H., Sekine, K.T.  Journal of Virology  97: e0046323

2. Efficient double-flowered gentian plant production using the CRISPR/Cas9 system. Nishihara, M., Hirabuchi, A., Goto, F., Watanabe, A., Yoshida, C., Washiashi, R., Odashima, M., Nemoto, K.   Plant Biotechnology 40: 229-236(link) 

3.  Preface to the special issue “Current Status and Future Prospects for the Development of Crop Varieties and Breeding Materials Using Genome Editing Technology”.  Nishihara, M. and Muranaka, T. Plant Biotechnology 40:181-184(link) 

4. Production of yellow-flowered gentian plants by genetic engineering of betaxanthin pigments. Nishihara, M., Hirabuchi, A., Goto, F., Nishizaki, Y., Uesugi, S., Watanabe, A., Tasaki, K., Washiashi, R., Sasaki, N. New Phytologist 240: 1177-1188 (link)

5. Identification of a new gibberellin receptor agonist, diphegaractin, by a cell-free chemical screening system. Nozawa, A., Miyazaki, R., Aoki, Y., Hirose, R., Hori, R., Muramatsu, C., Shigematsu,  Y., Nemoto, K., Hasegawa, Y., Fujita, K., Miyakawa, T., Tanokura,  M., Suzuki ,S., Sawasaki, T. Communications Biology 6: 448 (link)

6. Metabolic engineering of betacyanin in vegetables for anti-inflammatory therapy.   Saito, S., Nishihara, M., Kohakura, M.,  Kimura, K.,  Yashiro, T., Takasawa, S.. Arimura, G.  Biotechnology and Bioengineering 120: 1357-1365 (link)

2022

1. De novo transcriptome analysis reveals flowering-related genes that potentially contribute to flowering-time control in the Japanese cultivated gentian Gentiana triflora. Takase, T., Shimizu, M., Takahashi, S., Nemoto, K., Goto, F., Yoshida, C., Abe, A., Nishihara, M.  International Journal of Molecular Sciences 23: 11754 (link)

2. Methods to promote seed germination in the lacquer tree, Toxicodendron vernicifluum (Stokes) F.A. BarkleyNemoto, K., Watanabe, A., Yoshida, C., Nishihara, M. PLoS ONE 17: e0272665 (link)

3. Genome analysis revives a forgotten hybrid crop Edo-dokoro of the genus Dioscorea.  Natsume, S., Sugihara, Y., Kudoh, A., Oikawa, K., Shimizu, M., Ishikawa, Y., Nishihara, M., Abe, A., Innan, H., Terauchi, Plant & Cell Physiology 63: 1667–1678 (link)

4. Identification of candidate genes responsible for flower colour intensity in Gentiana triflora. Tasaki, K., Watanabe, A., Nemoto, K., Takahashi, S., Goto, F., Sasaki, N., Hikage, T., Nishihara, M.  Frontiers in Plant Science 13: 906879 (link).

5. Isolation and functional analysis of EPHEMERAL1-LIKE (EPH1L) genes involved in flower senescence in cultivated Japanese gentians.    Takahashi, S,   Yoshida, C.,   Takahashi, H., Nishihara, M.  International Journal of Molecular Sciences 23, 5608  (link) .

6. Calcium-dependent protein kinase 16 phosphorylates and activates the aquaporin PIP2;2 to regulate reversible flower opening in Gentiana scabra.   Nemoto, K., Niinae, T., Goto, F., Sugiyama, N., Watanabe, A., Shimizu, M., Shiratake, K., Nishihara, M. The Plant Cell 34: 2652–2670 (link)

7. Gentian FLOWERING LOCUS T orthologs regulate phase transitions: floral induction and endodormancy release. Takahashi, H., Nishihara, M., Yoshida, C., Itoh, K.   Plant Physiology 188: 1887-1899 (link)

8. ひとりではじめる　植物バイオテクノロジー入門　～組織培養からゲノム編集まで～「リンドウの形質転換およびゲノム編集」pp212-223　西原昌宏、国際文献社 (link)

9. ｢日本園芸リンドウにおけるゲノム編集技術の利用の現状と展望｣　西原昌宏  ゲノム編集作物：花卉の開発と新展開  月刊アグリバイオ増2022年８月号　北隆館 (link)　

2021

1. Post-transcriptional gene silencing of chalcone synthase gene CHS causes corolla lobe-specific whiting of Japanese gentian.  Ohta, Y., Atsumi, G., Yoshida, C., Takahashi, S., Shimizu, M. Nishihara, M., Nakatsuka, T.  Planta 255: 29 (link)

2. Identification and characterization of xanthone biosynthetic genes contributing to the vivid red coloration of red-flowered gentian. Sasaki, N., Nemoto, K., Nishizaki, Y., Sugimoto, N., Tasaki, K., Watanabe, A., Goto, F., Higuchi, A., Morgan, E., Hikage, T., Nishihara, M.  Plant Journal 107: 1711-1723  (link) 

3. Production of raspberry ketone by redirecting the metabolic flux to the phenylpropanoid pathway in tobacco plants. Koeduka, T., Takarada, S., Fujii, K., Sugiyama, A., Yazaki, K., Nishihara, M., Matsui, K.  Metabolic Engineering Communications 13: e00180 (link)

4. Additional betalain accumulation by genetic engineering leads to a novel flower color in lisianthus (Eustoma grandiflorum).   Tomizawa, E., Ohtomo, S., Asai, K., Ohta, Y., Takiue, Y., Hashumi, A., Nishihara, M., Nakatsuka, T.  Plant Biotechnology 38: 323-330 (link)

5. The rice wound-inducible transcription factor RERJ1 sharing same signal transduction pathway with OsMYC2 is necessary for defense response to herbivory and bacterial blight. Valea, I., Motegi, A., Kawamura, N., Kawamoto, K., Miyao, A., Ozawa, R., Takabayashi, J., Gomi, K., Nemoto, K., Nozawa, A., Sawasaki, T., Shinya, T., Galis, I., Miyamoto, K., Nojiri, H., Okada, K.  Plant Molecular Biology (in press) 

2020

1. Esterified carotenoids are synthesized in petals of carnation (Dianthus caryophyllus) and accumulate in differentiated chromoplasts.Iijima, L., Kishimoto, A., Ohmiya, A., Yagi, M., Okamoto, E., Miyahara, T., Tsujimoto, T., Ozeki, Y., Uchiyama, N., Hakamatsuka, T., Kouno, T., Cano, E.A., Shimizu, M.,  Nishihara, M. Scientific Reports 10: 15256 (link)

2. Morphological and cytological observations of corolla green spots reveal the presence of functional chloroplasts in Japanese gentian. Takahashi, S., Ozawa, S., Sonoike, K., Sasaki, K., Nishihara, M.   PLoS ONE 15: e0237173 (link) 

3. Molecular characterization of an anthocyanin-related glutathione S-transferase gene in Japanese gentian with the CRISPR/Cas9 system.  Tasaki, K., Yoshida, M., Nakajima, M., Higuchi, A., Watanabe, A., Nishihara, M.  BMC Plant Biology  20: 370 (link)

4. Soy and Arabidopsis receptor-like kinases respond to polysaccharide signals from Spodoptera species and mediate herbivore resistance. Uemura T., Hachisu M., Desaki Y., Ito A., Hoshino R., SanoY., Nozawa A., Mujiono K., Galis I., Yoshida A., Nemoto K., Miura S., Nishiyama M., Nishiyama C., Horito S., Sawasaki T., Arimura G.    Communications Biology 3: 224 (link)

5. Kurokawa, K., Kobayashi, J., Nemoto, K., Nozawa, A., Sawasaki, T., Nakatsuka, T., Yamagishi, M.  Expression of LhFT1, the Flowering Inducer of Asiatic Hybrid Lily, in the Bulb Scales. Frontiers in Plant Science  11: 570915 (link)

6. 高橋重一、西原昌宏 ｢新発見！リンドウは花弁の緑色斑点で光合成する｣ 学会誌 光合成研究 Vol30 (No3) 157-165.(link)

2019

1. Effects of knocking out three anthocyanin modification genes on the blue pigmentation of gentian flowers.  Tasaki, K., Higuchi, A., Watanabe, A., Sasaki, N., Nishihara, M. Scientific Reports 9: 15831 (link)

2. The Ring-type E3 Ubiquitin Ligase JUL1 Targets the VQ-motif Protein JAV1 to Coordinate Jasmonate Signaling. Mohamed R. M. Ali, Takuya Uemura, Abdelaziz Ramadan, Kyoko Adachi, Keiichirou Nemoto, Akira Nozawa, Ryosuke Hoshino, Hiroshi Abe, Tatsuya Sawasaki, Gen-ichiro Arimura. Plant Physiology 179: 1273-1284 (link)

3. Tyrosine kinase-dependent defense responses against herbivory in Arabidopsis. Miyamoto, T., Uemura, T., Nemoto, K., Daito, M., Nozawa, A., Sawasaki, T., Arimura, G.  Frontiers in Plant Science 10: 776 (Link)

4. Inhibition of post-transcriptional gene silencing of chalcone synthase genes in Petunia picotee petals by fluacrypyrim.     Ban, Y., Morita, Y., Ogawa, M., Higashi, K., Nakatsuka, T., Nishihara, M., Nakayama, M. Journal of Experimental Botany 70: 1513–1523 (Link)

5. The host stomatal density determines resistance to Septoria gentianae in Japanese gentian. Tateda, C., Obara, K., Abe, Y., Sekine, R., Nekozuka, S., Hikage, T., Nishihara, M., Sekine, K., Fujisaki, K.  Molecular Plant-Microbe Interactions 32: 428-436 (Link)

6.  Overexpression of geraniol synthase induces heat stress susceptibility in Nicotiana tabacum. Hamachi, A., Nishihara, M., Saito, S., Rim, H., Takahashi, H., Islam, M., Uemura, T., Ohnishi, T., Ozawa, R., Maffei, M.E., Arimura, G. Planta 249: 235-249 (Link)

7. Sugar composition in asparagus spears and its relationship to soil chemical properties. Takahashi, H., Yoshida, C., and Takeda, T.    Journal of Applied Glycoscience 66: 47-5 (Link)

2018

1. Application of the CRISPR/Cas9 system for modification of flower color in Torenia fournieri. Nishihara, M., Higuchi, A.,  Watanabe, A., Tasaki, K. BMC Plant Biology 18:331(Link) 

2. Biological effects of ion beam irradiation on perennial gentian and apple. Sasaki, N., Watanabe, A, Asakawa, T., Sasaki, M., Hoshi, N., Naito, Z., Furusawa, Y., Shimokawa, T., Nishihara, M. Plant Biotechnology 35: 249-257 (Link)

3. Carnation I locus has two chalcone isomerase genes involved in orange flower. Miyahara, T., Sugishita, N., Ishida-Dei, M., Okamoto, E, Kouno, T., Cano, E.A., Sasaki, N., Watanabe, A., Tasaki, K., Nishihara, M., Ozeki, Y.  Breeding Science 68: 481-487 (Link) 

4. Gtgen3A, a novel plant GH3 β-glucosidase, modulates gentio-oligosaccharide metabolism in Gentiana. Takahashi, H., Kikuchi-Fujisaki, S., Yoshida, C., Yamada, H., Yamashita, T., Konno, N., Takeda, T.  Biochemical Journal. 475: 1309-1322. (Link)

5. 植物のたくらみ ー香りと色の植物学」有村源一郎・西原昌宏  ベレ出版  (Link)

6. Development of basic technologies for improvement of breeding and cultivation of Japanese gentian.  Nishihara, M., Tasaki, K., Sasaki, N., Takahashi, H. Breeding Science 68: 14–24 (Link)

7.  Repressed expression of a gene for a basic helix-loop-helix protein causes a white flower phenotype in carnation. Totsuka, A., Okamoto, E., Miyahara, T., Kouno, T., Cano, E.A., Sasaki, N., Watanebe, A., Tasaki, K. Nishihara, M., Ozeki, Y. Breeding Science 68: 139–143 (Link)

8.  Identification of new abscisic acid receptor agonists using a wheat cell-free based drug screening system. Nemoto, K., Kagawa, M,, Nozawa, A., Hasegawa, Y., Hayashi, M., Imai, K., Tomii, K., Sawasaki, T.  Scientific Reports 8:4268 [Link] 

2017

1. Development of molecular markers for breeding of double flowers in Japanese gentian. Tasaki, K.,  Higuchi, A.,  Fujita, K., Watanabe, A., Sasaki, N.,  Fujiwara, K.,  Abe, H.,  Naito, Z., Takahashi, R., Hikage, T. and  Nishihara, M.  Molecular Breeding 37:33 [link] 

2. The use of metabolome analysis to identify the cause of an unexplained disease of Japanese gentians (Gentiana triflora). Takahashi, H., Abe, H., Fujita, K., Sekine, K. Metabolomics 13:51  [link] 

3. The 64-bp sequence containing GAAGA motif is essential for CaMV-35S promoter methylation in gentian.  Shimada, A., Okumura, A., Iwata, Y., Koizumia, N., Nishihara, M., Mishiba, K.  BBA - Gene Regulatory Mechanisms 1860: 861-869  [link] 

4. Tyrosine phosphorylation of the GARU E3 ubiquitin ligase promotes gibberellin signalling by preventing GID1 degradation.Nemoto, K., Ramadan, A., Arimura, G., Imai,K., Tomii, K., Shinozaki, K., Sawasaki, T.  Nature Communications 8:1004 [link] 

2016

1. Development of a Broad bean wilt virus 2-based expression vector for gentian. Tasaki,K., Atsumia,G, Nishihara, M. and Sekine, K. Scientia Horticulturae 201:279–286 [link] 

2. Functional characterization of duplicated B-class MADS-box genes in Japanese gentian. Nakatsuka, T., Saito, M. and Nishihara, M. Plant Cell Reports 35: 895-904  [link] 

3. CaMV-35S promoter sequence-specific DNA methylation in lettuce. Okumura, A.,   Shimada, A., Yamasaki, S., Horino, T., Iwata, Y., Koizumi, N., Nishihara, M., Mishiba, K. Plant Cell Reports 35: 43-51 [link] 

4. Metabolite profiling reveals the involvement of aberrant metabolic changes in Gentiana triflora seed showing poor germination. Takahashi, H., Fujita, K., Yoshida, C., Nishihara, M.  J. Hort. Sci. Biotech. 91:148–155 [link]